If you wish to submit spoligotype or VNTR-MIRU data for the SitVit database, please download the following excel file. Please fill it carefully and send it to Dr. Nalin Rastogi (nrastogi@pasteur-guadeloupe.fr)
Excel model file
Read Instructions to fill the excel file
How to fill the ModeleMIRU excel file :
Excel File FIELD |
Instructions |
Field Properties |
| Strain No. | Strain number only. If possible make it 5 digits long, e.g., write 00017 if your strain number is 17. | REQUIRED |
| Old Iso Number | Fill only if the same strain has already been submitted to the database (e.g. you are using it to submit new information on the same strain, such as MIRUs data for a strain for which only Spoligotyping data was submitted). | OPTIONAL |
| No. of isolates | If possible, mention only 1 isolate/patient (which means that if you have 15 isolates with the same pattern, but from different patients, please enter them separately, with individual isolate numbers for each). | REQUIRED |
| Year of isolation | As a 4 digit value, e.g. 1999, 2001 etc., if unknown put 0000 | REQUIRED |
| Mycobacterial Species | Must be one of the M. tuberculosis complex sub-species e.g., M. tuberculosis, M. africanum, M. bovis, M. caprae etc., (leave empty if identification was not done further than "M. tuberculosis complex") | OPTIONAL |
| Isolation Country | Country of isolation of the isolate as a 3 letter code available at
ftp://ftp.ripe.net/iso3166-countrycodes.txt (this item may need a subscription). However, these 3 letter codes are freely available
at a link by Wikipedia :
|
REQUIRED |
| Isolation City | The city of isolation (if not mentioned, the country capital will be used in the database). | OPTIONAL |
| Country of origin / Ethnic background of patient | If available, as a 3 letter code(same links as Isolation Country : for some countries, it may be considered unethical to give information on ethnic background. In this case, the country of origin may be a helpful indicator) | OPTIONAL |
| Sex / Age of patient | If available, sex as “M" or "F", age in "years" | OPTIONAL |
| Spoligotype 43 | Fill as follows |
AT LEAST ONE |
| MIRU12 | 12 digit MIRU values only (in the order MIRU 2-4-10-16-20-23-24-26-27-31-39- and 40) |
|
| ETRs (A to E) | 5 digit VNTRs representing Exact Tandem Repeats A, B, C, D, E | |
| Investigator | name of the investigator (Name + initials, e.g. "Kremer, K.") | REQUIRED |
| Laboratory Code | leave empty (will be filled by us) | LEAVE EMPTY |
| Publication | publication(s) referring to the isolate, otherwise leave empty. | OPTIONAL |
| Drug susceptibility | Fill as follow :
|
OPTIONAL |
| Comments | Specify drug resistances as asked above ; also provide any clinical and/or demographic information worth mentioning, e.g. HIV serology, any prevalent clusters, etc., this list is not exhaustive. | OPTIONAL |
Supervisor & Web Content Manager : Dr. Nalin RASTOGI
Validation : Dr. Thierry ZOZIO
Please note that the newly released SITVIT2 (an updated version of SITVITWEB) is now freely available at: http://www.pasteur-guadeloupe.fr:8081/SITVIT2/
Copyright © 2011, Institut Pasteur de la Guadeloupe. All rights reserved. |
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